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Science Scientific Investigation Q15
Passage III

Amphiprion percula, a species of clownfish, are kept in many home aquariums. Two experiments were conducted to determine how diet and stocking density (number of fish per liter of seawater, fish/L) affect the specific growth rate (SGR; percent increase in length per day, percent/day) in A. percula. Experiment 1 Each of 12 identical 15 L tanks received 10 L of seawater having a salinity of 33 parts per thousand (ppt), a temperature of 27°C, and a pH of 8.2. Salinity, temperature, and pH were kept constant over the course of the experiment. A. percula of similar lengths were selected, and their lengths were measured, in cm, with a ruler. Then they were equally distributed among the tanks at a stocking density of 1 fish/L. The tanks were then divided equally into 4 groups. For 4 months, each group was fed a different diet (Diets Q−T). Each group was fed the same mass of food 3 times daily. At the end of 4 months, the length of each fish was measured, in cm, with a ruler, and the SGR of each fish was calculated. The average SGR was then determined for each group (see Table 1). Table 1 Average SGR Diet (percent/day) Q 0.30 R 0.40 S 0.50 T 0.35 Experiment 2 The procedures for Experiment 1 were repeated except that each group was kept at a different stocking density, 0.5 fish/L, 1 fish/L, 2 fish/L, or 3 fish/L, and all fish were fed Diet T. At the end of 4 months, the average SGR was determined for each group (see Table 2). Table 2 Stocking density Average SGR (fish/L) (percent/day) 0.5 0.50 1 0.35 2 0.25 3 0.20 Tables adapted from João Chambel et al., “Effect of Stocking Density and Different Diets on Growth of Percula Clownfish, Amphiprion percula (Lacepede, 1802).” ©2015 by Springer.

Suppose that, in the experiments, 1 g of food were added to each tank at each feeding. A total of how many grams of food would have been placed into an individual tank each day?

  1. A

    1 g

  2. B

    3 g

  3. C

    12 g

  4. D

    36 g

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